InGelPermeation Chromatography process, the sample is poured into the column, which may include asemi-permeable membrane or a selective column designed to adsorbcertain size classes of substances. The column is primed before useand maintained at specific concentrations for a pre-set period oftime. The sample is recovered after retention time and analyzed on aGE Healthcare spectrophotometer.
Manyindustries employ this technique as a way of obtaining reliable dataon the composition of key chemicals and other materials that cannotbe detected by more conventional techniques. This is because thistechnique separates highly sensitive substances from the massbackground of the chromatogram. Both qualitative and quantitativeanalyses can be obtained from this method. Gel permeationchromatogram (GPC), a compound of chromatography, is one of the twogeneral-based GPC methods, which relies on the use of a column totrap sample particles on the surface of the column.
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