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Lipids are among the most important organic compounds in living organisms, where they function as cellular membrane building blocks as well as energy storage and signalling molecules. Lipid Analysis Devices is the science of determining individual lipid species on a large scale, and the underlying analytical technology used to identify and quantify the lipidome is typically mass spectrometry (MS). This review article provides an overview of the key steps in MS-based lipidomics workflows, such as sample preparation, liquid-liquid or solid-phase extraction, derivatization, chromatography, ion-mobility spectrometry, MS, and data processing using various software packages.

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