1. Science / Technology

Many derivatives of triphenylmethanol are important dyes

Disclaimer: This is a user generated content submitted by a member of the WriteUpCafe Community. The views and writings here reflect that of the author and not of WriteUpCafe. If you have any complaints regarding this post kindly report it to us.

Triphenylmethanol (also known as triphenylmethanol and TrOH) is an organic compound. It is a white crystalline solid, insoluble in water and petroleum ethers, but soluble in ethanol, ether and benzene. In strongly acidic solutions, it produces a strong yellow color due to the formation of stable “triphenylmethyl” carbon cations. Many derivatives of triphenylmethanol are important dyes.

Therefore, in this study, we examined the characteristics of triphenylmethanol pre-peaks and developed a novel HPLC-ECD based on triphenylmethanol voltammetric oxidation for the determination of theophylline in plasma. The concentration time curve of theophylline in rat plasma was obtained by a novel high performance liquid chromatography-electron capture method to determine the pharmacokinetic parameters.


All chemicals and solvents are reagent grade. triphenylmethanol (>97.0%) was purchased from Calbiochem (La Jolla, CA, USA). Acetonitrile (>99.8%, HPLC grade), LiClO4 (>98.5%), trichlorothane (>99%), isopropyl alcohol (>99.5%), theophylline (>99%), caffeine (>98.5%) and theobromine (>98%) from Wako Pure Chemical Industries Co. (Osaka, Japan). Uric acid (>98%) and 3-methylxanthine (>98%) were purchased from Tokyo Kasei Kogyo Co., LTD. (Tokyo, Japan). The water used is distilled and used

Theophylline measured the characteristics of the pre-peak

Linear sweep voltammetry of triphenylmethanol with and without theophylline was performed in acetonitrile solutions containing 0.54 mmol L−1 triphenylmethanol and 13mmol L−1 LiClO4 using PFC electrodes. The oxidation peak of +0.85V for Ag/AlgCl was observed on the voltammetry of Ag/AgCl scans from +0.05V to +1.0V (Figure 2, curve a). In the presence of theophylline, a new peak (oxidation pre-peak) appears at a positive potential lower than the oxidation peak (Figure 2, curve B-D).


Based on the triphenylmethanol pre-peak caused by the presence of theophylline on the voltamogram, a new electrochemical detection method was developed for the determination of theophylline by HPLC. The current HPLC-ECD method is highly selective for theophylline detection and can be determined within 10 minutes. The detection limit of theophylline was 0.55μmol L−1 with a linear range of 2.0 to 100μmol L1 (r > 0.999).


Welcome to WriteUpCafe Community

Join our community to engage with fellow bloggers and increase the visibility of your blog.
Join WriteUpCafe