After lactose and fat, Human Milk Oligosaccharide (HMOs) are the third most prevalent solid (dissolved, emulsified, or suspended in water) component. Depending on the stage of lactation, HMOs can range in concentration from 11.3 to 17.7 g/L (1.5 oz/gal to 2.36 oz/gal) in human milk. There are about 200 known Human Milk Oligosaccharides, which can be divided into fucosylated, sialylated, and neutral core HMOs based on their structural differences. Every mother's breast milk has a unique composition of Human Milk Oligosaccharide, which changes over the course of lactation. 2′-Fucosyllactose, which is present in human breast milk at a concentration of about 2.5 g/L, is the predominant oligosaccharide in 80% of all women. Other common oligosaccharides include lacto-N-tetraose, lacto-N-neotetraose, and lacto-N-fucopentaose. Numerous research have discovered it. that numerous aspects of lactation, including the mother's genetic secretor status and gestational length, affect the concentration of each unique human milk oligosaccharide (colostrum, transitional, mature, and late milk).

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