NGS or Next-Generation sequencing has been a game-changer for genomic research, making it possible to sequence entire genomes in a single day. With Next-Gen sequencing, scientists have access to an ultra-high throughput, scalable, and rapid method to answer a wide spectrum of genetic questions.

With the increasing usage of NGS, the requirements for high-quality and quantity DNA going into sequencing pipelines should also be kept in mind. Here are the ideal requirements for NGS.

NGS (Next-Gen Sequencing)

Next-Gen Sequencing refers to modern sequencing technology with which millions of DNA fragments can be sequenced in parallel. Once the short reads have been generated, bioinformatic analyses is used to piece these fragments together. In the process, the entire genome can be sequenced multiple times, resulting in accurate, high-depth data. 

The quality and quantity of DNA used for NGS are extremely important. And that requires an optimized DNA extraction pipeline that can be scaled easily.

HighPrep™Blood & Tissue DNA Kit utilizes proprietary magnetic beads chemistry and requires no phenol or chloroform extraction or alcohol precipitation and is suited for high throughput automation.

This extraction method eliminates the need for centrifugation or vacuum, thereby reducing stress on the target molecules, requiring lesser number of steps and reagents, and increasing ease of automation in 24, 96, and 384-well plates.

Magbio Genomics’ HighPrep™Blood and Tissue DNA Kit has been designed to isolate high-quality genomic DNA from 50-250 µL of a wide variety of samples –

• fresh or frozen whole blood
• buffy coat containing anticoagulants such as Citrate, EDTA and Heparin
• fresh or frozen animal tissues and cells
• lysate of tissues
• mouse tails
• cultured cells
• saliva
• buccal swabs

Whether you have a small number of samples or high throughput setting on automated workflow, you can isolate high quantity and quality of genomic DNA with a HighPrep™ Blood & Tissue DNA Kit that utilizes paramagnetic beads. The isolated gDNA can then be used in various genomic applications, such as amplification and enzymatic reactions.

 If you are looking for viral DNA/RNA extraction kit and RNA extraction kit manufacturers in the USA, call MagBio Genomics at (301) 302-0144 or get in touch at [email protected]. MagBio Genomics is the industry leader in magnetic bead-based products for nucleic acid isolation and DNA, cfDNA, and RNA extraction.