Next-generation sequencing (NGS) has been a game changer in understanding various diseases by allowing analyze genetic variation, expression and regulation,at scales never possible before. Nucleic acid, or DNA and RNA extraction, plays a vital role in NGS as the primary step for many downstream applications. For example, MagBio Genomics’ HighPrep™ Viral/Pathogen DNA/RNA Kit allows rapid isolation of viral, bacterial and fungal nucleic acids from whole blood, serum, plasma, saliva and other body fluids.
Magnetic beads-based DNA/RNA purification is one of most effective methods for extracting RNA and genomic, plasmid, and mitochondrial DNA. This rapid and efficient nucleic acid extraction doesn’t involve centrifugation, allowing intact longer fragments from genomic DNA. It also provides an alternative for automation of extraction from a large number of samples. It is easy to execute, and is a great choice for high-throughput applications, and high sample processing. Since it is virtually equipment free, magnetic beads based nucleic acid purification is also suited for use in low technological environments.
Modern, highly efficient Viral DNA/RNA Extraction kits offer faster purification of viral nucleic acids from cell–free samples such as serum, plasma and CSF. The kit contains a viral/pathogen lysis buffer, RDW buffer, nuclease-free water, Pro K solution, NBE, Mag-S1 particles, LES I and II.These kits use magnetic beads to bind viral RNA/DNA molecules under special conditions. The proteins and cellular debris are washed out. The resultant pure RNA and DNA are then eluted in nuclease-free water or low ionic strength buffer. The extracted RNA or DNA doesn’t need further purification to be used in downstream applications.
The HighPrep™ Viral/Pathogen DNA/RNA kit has been designed for effective and quick isolation of viral, bacterial and fungal nucleic acids from –
- whole blood
- serum
- plasma
- saliva
- other body fluids
- nasopharyngeal swabs soaked in virus transport media or other buffers
The HighPrepTM kit for DNA/RNA isolation enables efficient viral nucleic acid isolation. The extracted nucleic acid is suited to most downstream applications, such as –
- RT-qPCR
- RT-PCR
- PCR
- nucleic acid amplification
- cloning
- sequencing
- enzymatic reactions
These kits can be used both in -
- low throughput manual workflows, and
- can be adapted to most the liquid handling workstations
If you are looking for magnetic beads based, high-quality viral DNA/RNA extraction kit or AmpureXP alternative, call one of the most trusted RNA extraction kit manufacturers in USA, MagBio Genomics at (301) 302-0144.