COVID-19 and other respiratory virus tests have become extremely important across the globe. But it is important to ensure efficient RNA extraction from the samples collected. The samples are typically collected from the upper respiratory tract. The tests detect viral RNA via a reverse transcriptase polymerase chain reaction (RT-PCR) or genetic sequencing.
Swab samples have been considered the best due to their high sensitivity but these samples must be collected by trained professionals wearing full PPE. Saliva samples are a good alternative since they are easy to self-collect with sensitivity-comparable swab samples.
Extracting viral RNA from saliva and swab samples
- Spin column RNA extraction
This method involves sample lysis with a lysis buffer, adding alcohol to promote RNA binding to the column’s silica matrix, washing RNA in a wash buffer, and then elution to hydrate the RNA, and allowing dissociation from the column’s silica matrix.
- Magnetic Beads based RNA extraction
High-throughput magnetic beads-based RNA extraction kits, such as the HighPrep™ viral RNA kit by MagBio Genomics, allow rapid and reliable purification of nucleic acids, compared to spin columns. The general workflow includes sample lysis, binding RNA to the magnetic beads, washing, and RNA is finally eluted from the beads.
Choosing the best RNA extraction kit for your application can be challenging because of the availability of many options.
MagBio Genomics offers a number of extraction kits but your selection depends on your specific needs.
The HighPrep™ viral RNA kit is designed for extracting viral DNA in the following:
- RT-qPCR; RT-PCR, PCR
- One-Step RT-qPCR
- Virus detection, genotyping
- Viral load monitoring
The HighPrep™ Viral RNA Kit is designed for rapid and reliable extraction of viral RNA from serum, plasma, saliva, and other body fluids as well as nasopharyngeal swabs soaked in virus transport media or other buffers. This kit is highly efficient in viral nucleic acid isolation and the extracted RNA is suitable for direct use in most downstream applications such as one-step RT-qPCR, RT-PCR, PCR, nucleic acid amplification, cloning, sequencing, and enzymatic reactions. The kit can be used in low throughput manual workflows and is also adaptable to the majority of the liquid handling workstations available.
If you are looking for magnetic beads-based kits for nucleic acid isolation, and sample collection and stabilization tubes, call one of the most trusted RNA extraction kit manufacturers in the USA, MagBio Genomics at (301) 302-0144.