Recombinant Trypsin (Cell Culture Grade)

recombinant trypsin (Cell Culture Grade)

CAS: 9002-07-7

The product is a biochemical grade genetically engineered enzyme, does not contain excipients and enzyme inhibitors，such as DFP, PMSF and TLCK, Has the same activity and specificity as native Trypsin.The activity of this product is inhibited by serine protease inhibitors such as PMSF and TLCK, metal ion chelation agents such as EDTA.

The enzyme trypsin Application

For cell culture:

1. Vaccine production, cell culture 

2. Cell digestion by microcarrier method 

3. Mild digestion of stem cellsIsolation of various animal cells. 

For Recombinant proteins:

1. Recombinant insulin production 

2. Protein analysis.

trypsin enzym Recommended Method

Protein preparation: The protein was dissolved in a solution of 25 mM Tris-HCl, pH 8.0.

Enzyme preparation: The desired enzyme powder was weighed and dissolved in 10 mM HCl to give an enzyme solution concentration of 1-10 mg/ml.

Trypsin Enzym Transport and Storage Stability

Transportation stability: It is transported in an ice pack to keep it active.

Storage stability: The lyophilized powder can be stored for at least 24 months at -15°C below.

1 mM hydrochloric acid or 50 mM acetic acid was dissolved and stored at -15°C below, repeated freeze- thaw 10 times, no loss of activity. It is recommended that the storage should not exceed 7 days after dissolution at 2~8℃, and it should not exceed 30 days after storage at -15°C below.

Notice and Disclaimer

If the temperature exceeds 37 °C, this product may self-digest.

The product has moisture absorption and should be taken after room temperature balance.

The product is for research use only and should not be used for diagnosis or treatment of animals or humans.

For more information about trypsin enzyme is present in and source of trypsin enzyme, please feel free to contact us!

There are many Cosmetic Raw Material manufacturers and suppliers, but we are one of the best choices for you.

If you are looking for a reliable biopharmaceuticals manufacturing manufacturer, please contact us.