A cell lines authentication DNA test is an essential research tool. It has comprehensive applications in drug discovery, genetics, vaccine development, reconstructive drugs, cell biology, toxicity testing, screening, etc. With the DNA testing technology growing continuously, getting a DNA test for various reasons has become easier than ever, including a Cell Line Authentication Test in India.
Why is a Cell Line Authentication DNA Test Required?
The following are the three types of errors that can be detected through this test:
Cross Contamination: Environmental pollutants like dust & fungus can easily contaminate the cells under analysis. Maintaining a contamination-free surrounding in the lab and storage areas is essential to overcome this problem. However, despite the care, contamination of the cells may be inevitable. This type of test can assist you in accurately identifying such mistakes.
Misidentification: Often, a cell line of interest is misidentified at the source. If not detected, a scientist would have continued using the same cell line for his research and may not get the expected results. It is a common mistake while working with cell lines.
Genetic Drift: Among the most critical mistakes with a cell line is that the actual cell line may become genetically drifted with time. In such possibilities, the cells may lose their identity and become different cells of genetic alteration.
Researchers acknowledge that the latter is more harmful than the first two errors. One may be unaware that the cells are slowly changing and may continue getting inaccurate results.
When to Authenticate a Cell Line?
Cell lines authentication tests involve methods like isoenzyme analysis and karyotyping. However, these procedures were insufficient for distinguishing between different cell lines. STR profiling is an easy, low-cost, & reliable way for this test. A lab should get relevant information regarding its growth characteristics, culture, and origin, conditions if it receives a cell line. This test may be needed for the following conditions:
- When there is an alteration in the behavior of the cell
- When there is a risk of cross-contamination
- When new cell lines should be collected
- Before starting any new experiments
How to Authenticate a Cell Line?
Short Tandem Repeat (STR) profiling establishes a unique fingerprint of every human cell line. Microsatellites are about 3-10 base pairs long and are the hypervariable parts of fungi, bacteria, fish, reptiles & insects. STR profiling can differentiate between various individuals of the same species. The number of repetitions present at the STR loci in each individual differs and gives rise to a unique fingerprint. The ATCC/ANSI/standard ASN-0002 has mentioned STR profiling as the best human cell line authentication method.
DNA Forensics Laboratory Pvt. Ltd.- For Accurate Cell Line Authentication Tests
Getting Cell Lines Authentication in India can be a critical decision, provided the importance & sensitivity of a research or project. DNA Forensics Laboratory Pvt. Ltd. are NABL accredited lab offering accredited DNA testing services, including a Cell Line Authentication Test.
They use standardized methods for DNA tests. It includes detecting the misidentified, genetically drifted, cross-contaminated cells that invalidate research results. They use an exclusive model to produce repeated DNA for each human cell line for the analysis. Each new DNA profile stock is checked against the base profile. Their clients are from various esteemed biotechnology companies, research institutes, and pharmaceutical companies.
What Makes Them Best?
They carry out the cell line authentication at their advanced facility, equipped with advanced technology & industry-standard methodologies. They provide 100% accurate and dependable results. Authentication of cells becomes critical because the authenticity of the research results greatly depends on the technology.
They have the fastest turnaround times, offering this test report within 10-15 business days. They also offer sample collection at the address of their clients. They are the only DNA testing company for a legal DNA test in India for the Honorable court of law.
