Luminex screening and performance analysis detect color-coded polystyrene or superparamagnetic microbeads coated with antibodies specific to the target analyte. First, microbeads that recognize different target analytes are mixed and incubated with the test sample. After the microbeads capture the analyte, they are recognized by multiple biotin-labeled specific detection antibody mixtures, and then combined with streptavidin-PE fluorescein for detection. Luminex bead labeled antibody was used for detection, and the results reached similar levels to ELISA in terms of accuracy, precision, and sensitivity. This method can greatly reduce the consumption of biological samples when measuring multiple target molecules, save costs and measurement time, and make the analysis of correlations between multiple target molecules more accurate.
